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Title   Lymphokine - Activated Killer ( LAK ) 세포의 활성에 대한 Cytokine 의 효과 ( Effects of Cytokine Combinations on Lymphokine - Activated Killer ( LAK ) Cell Generation )
Publicationinfo   1993 Jan; 025(05): 751-760.
Key_word   Lymphokine-activated killer(LAK) cell activity, Cytokines
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Abstract   Lymphokine-activated killer cellsG.AK) have limited clinical activity against human cancers although they were active in vitro studies. The current study was undertaken to investigate the effects of various cytokines and its combinations on LAK cell generation in vitra to evaluate any synergism of cytokines on LAK cell induction. Purified mononuclear cells(MNC) obtained from peripheral blood of three healthy subjects were cultured for 4 days with various cytokines and then tested for LAK activity as assessed by lysis of Daudi in a 4 hour 51Cr release assay. The data are presented as per cent specific lysis or lytic units(LU30) per 10(7) effectors when possible. 1) In kinetic studies for LAK cell generation, maximal LAK activity against Daudi cells was generated after 4 days of culture with IL-2. LAK activity generation was IL-2 dose-dependent. IL-1 induced LAK activity lower than that by IL-2. IL-4 was inactive in inducing LAK activity. 2) Maximal interferon-induced LAK cytotoxicity occurred after 24 hr of culture using 1000 U /ml IFN-a and I 000 U/ml IFN-r. The magnitude of IFN-a-induced LAK activity was similar to that induced by IFN-r. IFN-induced LAK activity was transient and waned by 48 hr of culture. 3) IL-1 synergized with IL-2 in the induction of LAK activity. The most effective doses were l0 U/ml IL-2 with 100 U/ml IL-1. IL-4 inhibited LAK activity when added at the start of culture of lymphocytes with IL-2, but IL-4 slightly enhanced LAK activity in lymphocytes preactivated by IL-2. These results suggest that IL-2, IL-l, IFN-a, IFN-r, and probably IL-4 are important biological agents inducing LAK cell activity and combinations of IL-2 with IFNs, IL-1 or IL-4 could serve to increase LAK cell generation in vivo as was demonstrated in vitro.
저 자   성인환(In Hwan Seoung),최지영(Jee Young Choi),윤환중(Hwan Jung Yun),전의건(Eui Gun Chun),길준영(Jun Young Kil),조덕연(Deog Yeon Jo),김삼용(Sam Yong Kim),노흥규(Heung Kyu Ro)